8QJC

T6SS-linked Rhs repeat protein - Salmonella bongori Rhs-core domain with toxin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-07
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	71.443, 118.326, 169.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.620 - 2.491
R-factor	0.1921
Rwork	0.190
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.620	2.580
High resolution limit [Å]	2.491	2.491
Rmerge	0.227	1.079
Rmeas	0.232	1.103
Rpim	0.047	0.223
Number of reflections	50747	4813
<I/σ(I)>	17
Completeness [%]	99.1	95.18
Redundancy	24.59
CC(1/2)	0.994	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292.15	1 ul of protein solution (2.29 g/l) was mixed with 1 ul reservoir solution (0.1 M MES pH 7, 2% ethylene glycol, 7% PEG 8,000). A crystal was flash frozen without additional cryoprotectant.