8QJA

T6SS-linked Rhs repeat protein - Advenella mimigardefordensis VgrG-Rhs core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-01
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	66.239, 97.783, 215.006
Unit cell angles	85.69, 89.99, 76.89

Refinement procedure

Resolution	48.480 - 3.360
R-factor	0.2598
Rwork	0.257
R-free	0.31730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.568
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	3.480
High resolution limit [Å]	3.360	3.360
Rmerge	0.159	0.554
Rmeas	0.187	0.652
Rpim	0.098	0.341
Number of reflections	70262	7161
<I/σ(I)>	7.5
Completeness [%]	93.9	95.02
Redundancy	3.52
CC(1/2)	0.986	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292.15	Protein solution (1 ul, 13.8 mg/ml) was mixed with 1 ul of mother liquor (0.1 M TRIS pH 8.5, 45% PEP426, 400 mM KCl)