8QJ5

Crystal structure of the Levansucrase beta from Pseudomonas syringae pv. actinidiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	57.610, 100.630, 77.930
Unit cell angles	90.00, 96.06, 90.00

Refinement procedure

Resolution	29.300 - 1.630
R-factor	0.16256
Rwork	0.161
R-free	0.19025
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.812
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.400	61.400	1.670
High resolution limit [Å]	1.630	7.300	1.630
Rmerge	0.076	0.030	1.090
Rmeas	0.083	0.033	1.186
Number of reflections	109338	1256	8081
<I/σ(I)>	14.89
Completeness [%]	99.7
Redundancy	6.43		6.53
CC(1/2)	0.999	0.999	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	20% PEG 3350, 100mM sodium thiocianate