8QIS
CrPhotLOV1 light state structure 72.5 ms (70-75 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.540, 121.540, 46.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.780 - 2.900 |
| R-factor | 0.2151 |
| Rwork | 0.212 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.845 |
| Data reduction software | CrystFEL |
| Data scaling software | SCALEIT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.840 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 8744 | 849 |
| <I/σ(I)> | 6.64 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 427.81 | 249.9 |
| CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 293 | 100 mM sodium cacodylate at pH 6.5 and 1.0 M sodium citrate dibasic trihydrate |
