8QIL

CrPhotLOV1 light state structure 37.5 ms (35-40 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	121.540, 121.540, 46.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.780 - 2.550
R-factor	0.205
Rwork	0.202
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	CrystFEL
Data scaling software	SCALEIT
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.840	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	8227	793
<I/σ(I)>	6.87	0.73
Completeness [%]	100.0	100
Redundancy	424.02	263.2
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	293	100 mM sodium cacodylate at pH 6.5 and 1.0 M sodium citrate dibasic trihydrate