8QI9
CrPhotLOV1 dark state structure determined by serial synchrotron crystallography at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.540, 121.540, 46.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.770 - 1.870 |
| R-factor | 0.1621 |
| Rwork | 0.161 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.364 |
| Data reduction software | CrystFEL |
| Data scaling software | SCALEIT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.700 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 17122 | 1673 |
| <I/σ(I)> | 13.25 | 0.73 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 1959.27 | 310.4 |
| CC(1/2) | 1.000 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 293 | 100 mM sodium cacodylate at pH 6.5 and 1.0 M sodium citrate dibasic trihydrate |
