8QH2

Crystal structure of chimeric UAP1L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9163
Spacegroup name	P 41
Unit cell lengths	113.523, 113.523, 43.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.000 - 2.750
R-factor	0.2351
Rwork	0.233
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2-4158-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.970	2.900
High resolution limit [Å]	2.750	2.750
Number of reflections	14923	2141
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	0.997	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.49 M sodium phosphate monobasic monohydrate, 0.91 M potassium phosphate dibasic, pH 6.9