8QF5
Complex between N-lobe of Arc and nanobody E5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.03 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 34.500, 99.480, 138.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.830 - 1.500 |
R-factor | 0.1457 |
Rwork | 0.144 |
R-free | 0.19260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.095 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.045 | 2.496 |
Rmeas | 0.052 | 2.900 |
Number of reflections | 146561 | 14662 |
<I/σ(I)> | 10.8 | 0.6 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 3.8 | |
CC(1/2) | 0.999 | 0.194 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8, 0.01 M ZnCl2 and 20% w/v PEG 6000 |