8QF4

Complex between N-lobe of Arc and nanobody H11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.83
Spacegroup name	P 1 21 1
Unit cell lengths	41.820, 52.070, 44.220
Unit cell angles	90.00, 109.89, 90.00

Refinement procedure

Resolution	35.160 - 1.020
R-factor	0.1296
Rwork	0.129
R-free	0.16020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.060
High resolution limit [Å]	1.020	1.020
Rmerge	0.051	1.666
Rmeas	0.062	2.047
Number of reflections	168367	14404
<I/σ(I)>	9.3	0.7
Completeness [%]	94.2	80.8
Redundancy	2.9	2.8
CC(1/2)	0.998	0.233

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M potassium phosphate citrate pH 4.2, 0.2 M NaCl and 20% w/v PEG 8000