8QEX
Streptavidin variant with a cobalt catalyst for CH metal-catalyzed hydrogen-atom-transfer (M-HAT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 48.456, 80.534, 78.766 |
| Unit cell angles | 90.00, 103.92, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.900 |
| R-factor | 0.23014 |
| Rwork | 0.229 |
| R-free | 0.25416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.591 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.155 | 1.150 |
| Rpim | 0.079 | 0.590 |
| Number of reflections | 22733 | 2267 |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 7.1 | |
| CC(1/2) | 0.997 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.05 M Citric acid, 19 % w/v PEG 1000, 0.1 M Lithium sulfate, 0.05 M Sodium phosphate dibasic dihydrate |
