8QEH
Crystal structure of the G11 protein heterotrimer bound to FR900359 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.644, 95.813, 126.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.113 - 1.430 |
| Rwork | 0.140 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.800 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 163494 | 8016 |
| <I/σ(I)> | 16.5 | 0.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 44 | 46.7 |
| CC(1/2) | 1.000 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.09 M Na Acetate pH 4.5, 2.7 % PEG Smears Medium, 6.3 % MPD, 0.5% n-octyl-beta-D-Glucoside and 10 mM Zinc sulfate heptahydrate |
