8QE1
Crystal structure of human MAT2a bound to S-Adenosylmethionine and Compound 15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.422, 93.877, 116.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.290 - 1.095 |
| R-factor | 0.1593 |
| Rwork | 0.159 |
| R-free | 0.16440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.8 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.294 | 1.138 |
| High resolution limit [Å] | 1.095 | 1.096 |
| Rmerge | 0.043 | 0.318 |
| Rmeas | 0.047 | 0.386 |
| Rpim | 0.018 | 0.214 |
| Total number of observations | 20642 | |
| Number of reflections | 137345 | 6863 |
| <I/σ(I)> | 20 | 3.1 |
| Completeness [%] | 89.7 | |
| Redundancy | 6.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 8-12 % PEG 8000, 8-12 % ethylene glycol, 0.1 M Hepes pH 8.0 |
