8QDA
Wdyg1p Ser256DHA (PMSF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.630, 88.470, 122.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.400 - 1.850 |
R-factor | 0.14845 |
Rwork | 0.147 |
R-free | 0.17961 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 1.175 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.052 | 0.538 |
Number of reflections | 79470 | 11549 |
<I/σ(I)> | 15.8 | 2.7 |
Completeness [%] | 99.3 | 99.7 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | 100 mM tris pH 9.2, 22% PEG 3350 |