8QD2
Ayg1p in complex with 1,3-Dihydroxynaphthalene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.470, 107.480, 92.510 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.750 |
| R-factor | 0.22909 |
| Rwork | 0.229 |
| R-free | 0.23865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.211 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.109 | 0.614 |
| Number of reflections | 159406 | 24753 |
| <I/σ(I)> | 6.5 | 2.1 |
| Completeness [%] | 94.8 | 96.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM K/Na tartrate, 25% PEG 3350 |
