8QD1
Ayg1p from A. fumigatus catalyzes polyketide shortening in the biosynthesis of DHN-melanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.830, 108.480, 91.890 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 46.710 - 1.700 |
| R-factor | 0.16757 |
| Rwork | 0.166 |
| R-free | 0.19864 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.524 |
| Number of reflections | 179315 | 28397 |
| <I/σ(I)> | 9.6 | 2.4 |
| Completeness [%] | 97.2 | 97.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100-200 mM K/Na tartrate, 15-26% PEG 3350 |
