8QBB
Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture +PIP2/-sterol (DOPC, DOPE, DOPS, PI(4,5)P2 50:20:20:10)
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.86 |
Software | PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |