8QB7
Pil1 in native eisosome lattice bound to plasma membrane microdomain
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Software | RELION,PHENIX,cryoSPARC,Gctf,UCSF Chimera,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |