8QB5
Crystal structure of apo-GltTk obtained with in meso crystallization (P6322 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 94.240, 94.240, 244.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.120 - 3.200 |
| R-factor | 0.2934 |
| Rwork | 0.292 |
| R-free | 0.31240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.322 | 3.968 |
| Number of reflections | 10342 | 1083 |
| <I/σ(I)> | 12.95 | 1.14 |
| Completeness [%] | 92.6 | 0.999 |
| Redundancy | 36.9 | 39.1 |
| CC(1/2) | 0.998 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.9 | 293 | 1 M ammonium sulfate 100 mM sodium acetate |
