8QAM
vaccinia virus Uracil DNA glycosidase mutant I197K-V200E-L204K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 4 |
| Unit cell lengths | 161.668, 161.668, 39.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.320 |
| R-factor | 0.13129 |
| Rwork | 0.130 |
| R-free | 0.16463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.118 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.420 | 1.340 |
| High resolution limit [Å] | 1.318 | 1.318 |
| Rmerge | 0.050 | 1.134 |
| Number of reflections | 119652 | 5311 |
| <I/σ(I)> | 23.4 | |
| Completeness [%] | 99.5 | 90.2 |
| Redundancy | 13.29 | |
| CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22 % PEG4000, 100 mM LiSO4, 100 mM Hepes pH 7.5 |
