8QA7
Crystal structure of HDAC6 catalytic domain 2 from zebrafish in complex with buffer component.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.390, 92.373, 96.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.630 - 1.470 |
| R-factor | 0.21824 |
| Rwork | 0.217 |
| R-free | 0.23953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.630 | 1.590 |
| High resolution limit [Å] | 1.470 | 1.510 |
| Number of reflections | 113964 | 15735 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.999 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M NH4Cl, 20% PEG3350, 2 mM NaAc 10 mg/ml protein mixed 1:1 with well solution Cryo 20% glycerol + well solution |
