8QA1
Cryo-EM structure of Cx26 solubilised in LMNG - Hemichannel classification NFlex conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.3 |
| Number of Particle | 7652471 |
| Software | RELION,CTFFIND,Coot,PHENIX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
