8Q9R

Crystal structure of MADS-box/MEF2D N-terminal domain bound to dsDNA and HDAC9 deacetylase binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-11
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	57.391, 60.042, 77.108
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	47.360 - 2.250
R-factor	0.2251
Rwork	0.223
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.320
High resolution limit [Å]	2.250	2.250
Rmerge		1.103
Number of reflections	25008	2276
<I/σ(I)>	9	1
Completeness [%]	99.7
Redundancy	3.6
CC(1/2)	0.998	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350)