8Q92
P301S Tau Filaments from the Brains of PS19 Transgenic Mouse Line
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.05 |
| Software | RELION,Servalcat,EPU,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
