8Q8K

KI Polyomavirus LTA NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	I 1 2 1
Unit cell lengths	107.237, 85.696, 139.064
Unit cell angles	90.00, 95.96, 90.00

Refinement procedure

Resolution	19.880 - 2.700
R-factor	0.2143
Rwork	0.212
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.880	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.050	0.520
Rmeas	0.070	0.670
Rpim	0.040	0.430
Number of reflections	34296	4564
<I/σ(I)>	8.9	1.4
Completeness [%]	99.2	99.3
Redundancy	2.4	2.3
CC(1/2)	1.000	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2 M NaCl, 0.1 M Tris pH 8.0, 16 % PEG4000. Protein stock pre-treated with 5:1 molar ratio IMPa: ivermectin