8Q8I
AO75L in complex with a synthetic trisaccharide acceptor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979181 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.579, 50.456, 51.543 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 62.900 - 1.770 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.18_3855: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.900 | 2.110 |
| High resolution limit [Å] | 1.769 | 1.796 |
| Number of reflections | 12052 | 604 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 85.2 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.986 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.1 | 291 | 0.1M TRIS; 0.1M BICINE, pH 8.1 37.5% Morpheus Precipitant mix4 0.03M CaCl2;0.03M MgCl2 |
