8Q8A
Crystal structure of Apis mellifera glutathione transferase delta 1, mutant M126L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM07 |
Synchrotron site | ESRF |
Beamline | BM07 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.474, 77.474, 81.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.700 - 1.750 |
R-factor | 0.2149 |
Rwork | 0.213 |
R-free | 0.24330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.760 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.060 | 1.590 |
Rmeas | 0.062 | 1.661 |
Rpim | 0.017 | 0.476 |
Number of reflections | 25703 | 1385 |
<I/σ(I)> | 30.2 | 1.7 |
Completeness [%] | 100.0 | |
Redundancy | 12.9 | |
CC(1/2) | 0.998 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG4000, 0.1M tri-sodium citrate pH 5.6, 0.2M ammonium sulfate |