8Q89
Crystal structure of Apis mellifera glutathione transferase delta 1 in a covalent dimeric state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97856 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.971, 77.971, 81.455 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.660 - 2.050 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.027 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.660 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.219 | 1.017 |
Rmeas | 0.223 | 1.036 |
Rpim | 0.044 | 0.198 |
Number of reflections | 16353 | 1220 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 100.0 | |
Redundancy | 26.6 | |
CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 16% PEG4000, 10% 2-Propanol, 0.1M HEPES pH 7.5, 0.2M Ammonium Sulfate |