8Q7I
PCNA from Chaetomium thermophilum in complex with Fen1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-03-20 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 87.354, 87.354, 214.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.860 - 1.950 |
R-factor | 0.17275 |
Rwork | 0.171 |
R-free | 0.20376 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.101 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.860 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.070 | 0.182 |
Rmeas | 0.074 | 0.193 |
Rpim | 0.023 | 0.065 |
Total number of observations | 247082 | 13994 |
Number of reflections | 23349 | 1631 |
<I/σ(I)> | 23.8 | 11.1 |
Completeness [%] | 99.9 | |
Redundancy | 10.6 | 8.6 |
CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 50mM Tris-HCl pH8.0, 150mM NaCl |