8Q7D
Neck of phage 812 after tail contraction (C12)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.07 |
Number of Particle | 32222 |
Software | Gautomatch,ISOLDE,RELION,SerialEM,Gctf,Coot,PHENIX |
Symmetry | C12 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |