8Q78

Structure of the FP specific VHH TPP-3077

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	45.727, 53.611, 100.770
Unit cell angles	90.03, 90.02, 64.79

Refinement procedure

Resolution	27.620 - 1.225
Rwork	0.168
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.620	1.269
High resolution limit [Å]	1.225	1.225
Rmerge	0.061	0.378
Number of reflections	245404	9517
<I/σ(I)>	16.05
Completeness [%]	84.0
Redundancy	6.7
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	Morpheus Screen I, condition A4