8Q6W

LSSmOrange - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-24
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.541870
Spacegroup name	P 31
Unit cell lengths	68.090, 68.090, 41.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.040 - 1.430
R-factor	0.13587
Rwork	0.134
R-free	0.18076
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.852
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.400	4.170	1.400
Rmerge	0.079	0.039	1.295
Rmeas	0.084	0.041	1.391
Number of reflections	41808	1583	6633
<I/σ(I)>	14.55
Completeness [%]	99.6
Redundancy	9.9
CC(1/2)	0.999	0.999	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	291	100 mM Sodium acetate 50 % v/v Polyethylene glycol 400 200 mM Lithium sulfate