8Q6W
LSSmOrange - Directionality of Optical Properties of Fluorescent Proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.541870 |
| Spacegroup name | P 31 |
| Unit cell lengths | 68.090, 68.090, 41.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.040 - 1.430 |
| R-factor | 0.13587 |
| Rwork | 0.134 |
| R-free | 0.18076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.852 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 4.170 | 1.400 |
| Rmerge | 0.079 | 0.039 | 1.295 |
| Rmeas | 0.084 | 0.041 | 1.391 |
| Number of reflections | 41808 | 1583 | 6633 |
| <I/σ(I)> | 14.55 | ||
| Completeness [%] | 99.6 | ||
| Redundancy | 9.9 | ||
| CC(1/2) | 0.999 | 0.999 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 100 mM Sodium acetate 50 % v/v Polyethylene glycol 400 200 mM Lithium sulfate |
