8Q67

Crystal structure of a homohexameric MCM from M. acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	228.699, 127.488, 177.038
Unit cell angles	90.00, 91.71, 90.00

Refinement procedure

Resolution	57.150 - 2.590
R-factor	0.2311
Rwork	0.231
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.491
Data reduction software	X-Area
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.150	2.640
High resolution limit [Å]	2.590	2.590
Rmerge	0.106
Rmeas	0.115
Rpim	0.044
Number of reflections	157088	7646
<I/σ(I)>	11.3
Completeness [%]	99.9	98.2
Redundancy	6.8
CC(1/2)	0.998	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.03 M NPS, 0.1 M MOPS/HEPES pH 7.5, 10 % (w/v) PEG 20,000, 20 % (v/v) PEG MME 550