8Q65
Mycobacterium smegmatis inosine monophosphate dehydrogenase (IMPDH) ATP-bound form
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.54 |
| Number of Particle | 2754934 |
| Software | PHENIX,Coot,ISOLDE,SerialEM,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING ONLY |
