8Q5O
N-terminal domain of restriction endonuclease Eco15I with tetra-methylated target DNA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.995, 95.995, 96.307 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.998 - 2.330 |
Rwork | 0.264 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.998 | 2.413 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.110 | 1.294 |
Rmeas | 0.113 | 1.327 |
Rpim | 0.026 | 0.290 |
Number of reflections | 22349 | 2202 |
<I/σ(I)> | 20.04 | 4.33 |
Completeness [%] | 99.8 | 99.68 |
Redundancy | 19.6 | 20.6 |
CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M HEPES, pH 6.5, 0.02 M CaCl2, 35 % pentaerythritol ethoxylate |