8Q5N
Apo form of restriction endonuclease NhoI.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.480, 58.760, 75.590 |
Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
Resolution | 38.170 - 2.650 |
R-factor | 0.2241 |
Rwork | 0.222 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.801 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.250 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.113 | 0.830 |
Rmeas | 0.122 | 0.900 |
Rpim | 0.047 | 0.047 |
Number of reflections | 9793 | 990 |
<I/σ(I)> | 14.57 | 2.4 |
Completeness [%] | 99.2 | 99.6 |
Redundancy | 6.7 | |
CC(1/2) | 0.997 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.1M Tris-BICINE, 20 % v/v Ethylene glycol, 10 % w/v PEG 8000; 0.02 M, 1,6-hexanediol, 0.02 M 1-butanol, 0.02 M 1,2-propanediol, 0.02 M 2-propanol, 0.02 M 1,4-butanediol, 0.02 M 1,3-propanediol |