8Q5M

N-terminal domain of restriction endonuclease Eco15I in the absence of DNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.921
Spacegroup name	P 21 21 21
Unit cell lengths	65.691, 109.710, 113.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.040 - 2.660
R-factor	0.259
Rwork	0.258
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.040	2.755
High resolution limit [Å]	2.660	2.660
Rmerge	0.038	0.898
Rmeas	0.041	0.963
Rpim	0.015	0.342
Number of reflections	24304	2390
<I/σ(I)>	29.39	2.08
Completeness [%]	99.8	99.92
Redundancy	8	7.8
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	3 M NaCl, 0.1 M MES, 10 mM NaSCN as additive