8Q4V

Crystal structure of YTHDC1 in complex with Compound 37 (ZA_356)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.800, 103.840, 41.910
Unit cell angles	90.00, 104.85, 90.00

Refinement procedure

Resolution	40.510 - 1.360
R-factor	0.208
Rwork	0.207
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.514	1.450
High resolution limit [Å]	1.360	1.360
Number of reflections	67659	10701
<I/σ(I)>	13.51
Completeness [%]	97.0
Redundancy	3.42
CC(1/2)	0.998	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5