8Q4U
Crystal structure of YTHDC1 in complex with Compound 36 (ZA_540b)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.100, 103.290, 42.160 |
Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
Resolution | 38.700 - 1.370 |
R-factor | 0.213 |
Rwork | 0.212 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.854 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 1.450 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 68786 | 10882 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 98.4 | |
Redundancy | 3.37 | |
CC(1/2) | 0.996 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |