8Q4I
The crystal structure of human chloride intracellular channel protein 5 delta 57-68
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-02 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.886 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 130.128, 220.832, 45.023 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.560 - 2.100 |
R-factor | 0.2333 |
Rwork | 0.232 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.713 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.023 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.054 | 0.684 |
Number of reflections | 38486 | 3926 |
<I/σ(I)> | 19.1 | 1.4 |
Completeness [%] | 99.8 | |
Redundancy | 16.7 | |
CC(1/2) | 0.999 | 0.815 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 7.0, 30% Polyethylene glycol 3,350 |