8Q3L

Human Gamma-D Crystallin R36S fresh serial crystallographic structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	53.600, 83.700, 100.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.660 - 2.100
R-factor	0.20624
Rwork	0.204
R-free	0.25783
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.450
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.660	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	50856	2844
<I/σ(I)>	3.87	0.95
Completeness [%]	99.9
Redundancy	26.7
CC(1/2)	0.974	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.8	298	50 mM phosphate buffer pH 7.8, 20 mM DTT