8Q3A

Crystal structure of YTHDC1 in complex with Compound 22 (ZA_393)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	40.060, 103.530, 42.080
Unit cell angles	90.00, 105.07, 90.00

Refinement procedure

Resolution	40.630 - 1.430
R-factor	0.2174
Rwork	0.216
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.636	1.510
High resolution limit [Å]	1.430	1.430
Number of reflections	59960	9262
<I/σ(I)>	12.6
Completeness [%]	97.3
Redundancy	3.46
CC(1/2)	0.997	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5