8Q38

Crystal structure of YTHDC1 in complex with Compound 19 (ZA_347)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.660, 103.890, 41.980
Unit cell angles	90.00, 104.33, 90.00

Refinement procedure

Resolution	40.670 - 1.420
R-factor	0.209
Rwork	0.208
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.676	1.500
High resolution limit [Å]	1.420	1.420
Number of reflections	60939	9789
<I/σ(I)>	10.27
Completeness [%]	97.3
Redundancy	3.36
CC(1/2)	0.994	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5