8Q37

Crystal structure of YTHDC1 in complex with Compound 18 (ZA_312)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.950, 103.550, 42.230
Unit cell angles	90.00, 104.73, 90.00

Refinement procedure

Resolution	38.640 - 1.280
R-factor	0.2118
Rwork	0.211
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.640	1.360
High resolution limit [Å]	1.280	1.280
Number of reflections	83615	13331
<I/σ(I)>	10.85
Completeness [%]	97.6
Redundancy	3.41
CC(1/2)	0.997	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5