8Q36

Structure of Nucleosome Core with a Bound Metallopeptide Conjugate (Foamy Virus GAG Peptide-Au[I] Compound)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-09-03
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.04
Spacegroup name	P 21 21 21
Unit cell lengths	107.194, 109.563, 183.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.828 - 2.604
Rwork	0.213
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.620
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	2.740
High resolution limit [Å]	2.600	2.600
Number of reflections	62688	5679
<I/σ(I)>	19.6
Completeness [%]	93.6
Redundancy	12.5
CC(1/2)	0.999	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Buffers containing MnCl2, KCl and K-cacodylate [pH 6.0].