8Q2Y

Crystal structure of YTHDC1 in complex with Compound 11 (ZA_572)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.910, 104.160, 41.880
Unit cell angles	90.00, 104.81, 90.00

Refinement procedure

Resolution	40.490 - 1.710
R-factor	0.2275
Rwork	0.226
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.934
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.490	1.810
High resolution limit [Å]	1.710	1.710
Number of reflections	35511	5668
<I/σ(I)>	17.07
Completeness [%]	98.9
Redundancy	3.4
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5