8Q2W

Crystal structure of YTHDC1 in complex with Compound 8 (CS_01)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.870, 103.070, 42.030
Unit cell angles	90.00, 104.75, 90.00

Refinement procedure

Resolution	38.560 - 1.410
R-factor	0.192
Rwork	0.191
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.560	1.500
High resolution limit [Å]	1.410	1.410
Number of reflections	61956	9911
<I/σ(I)>	9.88
Completeness [%]	98.9
Redundancy	3.35
CC(1/2)	0.997	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5