8Q2U
Crystal structure of YTHDC1 in complex with Compound 6 (ZA_308)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.000, 103.730, 42.100 |
Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
Resolution | 40.690 - 1.360 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.23240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.690 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Number of reflections | 69363 | 11028 |
<I/σ(I)> | 11.69 | |
Completeness [%] | 96.6 | |
Redundancy | 3.38 | |
CC(1/2) | 0.998 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |