8Q2T

Crystal structure of YTHDC1 in complex with Compound 5 (ZA_236)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	40.190, 103.700, 42.220
Unit cell angles	90.00, 105.29, 90.00

Refinement procedure

Resolution	38.770 - 1.410
R-factor	0.2226
Rwork	0.222
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.718
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.770	1.490
High resolution limit [Å]	1.410	1.410
Number of reflections	62249	9851
<I/σ(I)>	16.22
Completeness [%]	96.8
Redundancy	3.39
CC(1/2)	0.997	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5