8Q2S

Crystal structure of YTHDC1 in complex with Compound 4 (ZA_232)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.910, 103.680, 42.090
Unit cell angles	90.00, 105.12, 90.00

Refinement procedure

Resolution	40.630 - 1.410
R-factor	0.1985
Rwork	0.197
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.879
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.630	1.490
High resolution limit [Å]	1.410	1.410
Number of reflections	62590	9875
<I/σ(I)>	15.16
Completeness [%]	97.9
Redundancy	3.45
CC(1/2)	0.996	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5