8Q2R

Crystal structure of YTHDC1 in complex with Compound 3 (ZA_431)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	40.010, 103.520, 41.900
Unit cell angles	90.00, 104.57, 90.00

Refinement procedure

Resolution	40.550 - 1.600
R-factor	0.2094
Rwork	0.208
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.556	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	42926	6762
<I/σ(I)>	8.15
Completeness [%]	99.0
Redundancy	3.37
CC(1/2)	0.992	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5